WebABSTRACT Diphtheria toxin (DTx) is an extremely po-tent inhibitor of protein synthesis. It is secreted as a linear polypeptide, which is cleaved to produce disulfide-linked A and B fragments. Fragment A, the inhibitor of protein synthe-sis, requires fragment B, the recognition subunit, for entry into intact cells. Fragment B has been proposed ... WebIron dependent repressor. In molecular biology, the iron dependent repressors are a family of bacterial and archaeal transcriptional repressors . At their N-terminus they contain a dtxR-type HTH domain. This is a DNA -binding, winged helix-turn-helix (wHTH) domain of about 65 amino acids present in metalloregulators of the dtxR/mntR family. The ...
Mechanism of Insertion of Diphtheria Toxin: Peptide Entry …
WebDiphtheria toxin (DTx) provokes extensive internucleosomal degradation of DNA before cell lysis. The possibility that DNA cleavage stems from direct chromosomal attack by … Diphtheria toxin is an exotoxin secreted by mainly by Corynebacterium diphtheriae but also by Corynebacterium ulcerans and Corynebacterium pseudotuberculosis. the pathogenic bacterium that causes diphtheria. The toxin gene is encoded by a prophage called corynephage β. The toxin … See more Diphtheria toxin is a single polypeptide chain of 535 amino acids consisting of two subunits linked by disulfide bridges, known as an A-B toxin. Binding to the cell surface of the B subunit (the less stable of the two subunits) allows the … See more Diphtheria toxin is extraordinarily potent. The lethal dose for humans is about 0.1 μg of toxin per kg of body weight. Death occurs through necrosis of the heart and liver. Diphtheria toxin has also been associated with the development of myocarditis. … See more The drug denileukin diftitox uses diphtheria toxin as an antineoplastic agent. Resimmune is an immunotoxin that is in clinical trials in See more Similar to other A-B toxins, diphtheria toxin is adept at transporting exogenous proteins across mammalian cell membranes, which are usually impermeable to … See more 1. Processing 2. The toxin binds to heparin-binding epidermal growth factor precursor (HB-EGF). 3. The complex undergoes endocytosis by the host cell. 4. Acidification inside the endosome induces translocation of the A subunit into the cytosol. See more Diphtheria toxin was discovered in 1888 by Émile Roux and Alexandre Yersin. In 1890, Emil Adolf von Behring developed an anti-toxin based on the blood of horses immunized with attenuated bacteria. In 1951, Freeman found that the toxin gene was not encoded … See more 1. ^ A prophage is a virus that has inserted itself into the genome of the host bacterium. See more child study center brooklyn ny
The maternal antibody against diphtheria, tetanus and pertussis …
WebDiphtheria toxin (DTx) is a well-known bacterial toxin inducing various cytotoxic effects. Mainly, catalytic fragment inhibits protein synthesis, induces cytotoxicity, and depolymerizes actin ... WebDiphtheria toxin (DTx) receptor (DTR)-mediated conditional cell ablation in transgenic mice is a powerful tool to analyze cell function in vivo. Transgenic mice with cell-specific expression of the human DTR have been developed that allow conditional depletion of these cells in vivo through administration of the toxin. We have performed a ... Webthe ability of transmembrane helix 1 of diphtheria toxin to bind to COPI complex appears to be the essential feature for catalytic domain delivery to the cytosol. ... contributing to toxin efficacy; DTx was shown to degrade double-stranded DNA by non-processive, endonucleolytic attack, without apparent specificity for nucleotide sequence. ... gp at bankstown