Granuphilin c2a

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August undefined, 2024

NMR analysis of the granuphilin-C2A domain interacting with PI(4,5)P2-nanodisc demonstrates that, besides residues within the concave groove that are responsible for PI(4,5)P2 head group binding, the strand β5 was also affected. This region may be involved in direct binding to membrane and/or undergo conformation changes upon membrane binding. WebApr 1, 2016 · Furthermore, granuphilin forms a 180-nm cluster at the site of each docked granule, along with granuphilin-interacting Rab27a and Munc18-1 clusters. These …

The C2 domains of granuphilin are high-affinity sensors …

WebJan 13, 2015 · Another example is the study of the interaction of granuphilin-C2A domain with phosphatidylinositol 4, 5-bisphosphate [PI(4, 5)P 2 ] head group and PI(4, 5)P 2 … WebDec 12, 2013 · Proteins containing C2 domains are the sensors for Ca 2+ and PI(4,5)P 2 in a myriad of secretory pathways. Here, the use of a free-mounting system has enabled us to capture an intermediate state of Ca 2+ binding to the C2A domain of rabphilin 3A that suggests a different mechanism of ion interaction.Structural comparisons between C2A … inc. village of valley stream ny 11580

Multivalent lipid targeting by the calcium-independent …

WebInsights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2 MLA Chanjuan Wan, et al. "Insights Into the Molecular Recognition of the Granuphilin C2a … WebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma … WebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before exocytosis. ... in cabinet drawer led lighting

The C2 domains of granuphilin are high-affinity sensors for plasma ...

Aml A Alnaas , Abena Watson-Siriboe , Sherleen Tran , Mikias …

WebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of this study was to determine membrane-binding mechanisms, affinities, and kinetics of both granuphilin C2 domains using fluorescence spectroscopic techniques. WebGranuphilin is a membrane binding protein responsible, in part, for docking insulin secretory vesicles to the plasma membrane. Granuphilin, also known as synaptotagmin … inc. wax figuresWeband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the cluster contains other basic residues that impart a positively charged electrostatic surface (Fig. 1). The Slp-4 C2A domain plays a critical role in targeting its in cabinet drying racks

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Granuphilin c2a

WebMay 31, 2024 · Synaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before … WebMay 29, 2024 · Multivalent lipid targeting by the calcium-independent C2A domain of Slp-4/granuphilin Aml A Alnaas 1, Abena Watson-Siriboe 1, Sherleen Tran , Mikias Negussie, Jack A. Henderson , J. Ryan Osterberg 1, Nara Lee Chon , Julianna Oviedo,1 Tatyana Lyakhova1, Cole Michel2, Nichole Reisdorph 2, Richard Reisdorph , Colin T. Shearn3, …

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WebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of … WebSep 1, 2014 · The granuphilin C2A domain shows significant protein-to-membrane FRET when added to liposomes closely resembling the lipid composition of the plasma membrane cytosolic leaflet (Corbin et al., 2007, Voelker, 2008).Since some C2 domains are known to dock preferentially to PIP x lipids, such physiologically relevant lipid mixtures …

WebHHMI’s Janelia Research Campus in Ashburn, Virginia, cracks open scientific fields by breaking through technical and intellectual barriers. Our integrated teams of lab scientists … WebJan 27, 2015 · Granuphilin C2A has similar affinity for liposomes containing either 24% PS or 2% PIP2, but affinity increases ∼100-fold in the presence of both target lipids. Binding site(s) for the two lipids ...

WebCorvallis Veterinary Hospital. Jul 2007 - Sep 20092 years 3 months. 1543 NW 9th St. Corvallis, OR 97330. As part of a small veterinary clinic I gained proficiency in a diverse set of skills ... WebJun 3, 2024 · Synaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before …

Weband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the …

WebRab27a and Rab27b have recently been recognized to play versatile roles in regulating the exocytosis of secretory granules and lysosome-related organelles by using multiple effector proteins. However, the precise roles of these effector proteins in particular cell types largely remain uncharacterized, except for those in pancreatic β cells and in melanocytes. Here, … inc. vs corporationWebFeb 15, 2006 · Synaptotagmin-like protein 4-a (Slp4-a)/granuphilin-a is specifically localized on dense-core vesicles in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. However, the precise molecular mechanism of its inhibitory effect on exocytosis has never been … inc. waterford twpWebC2 Education is the fastest-growing test prep and tutoring center in the country. Use our Center Locator to help find a center near you and get started! in cabinet dual trash binWebFeb 11, 2024 · Seventy percent of the world’s internet traffic passes through all of that fiber. That’s why Ashburn is known as Data Center Alley. The Silicon Valley of the east. The … in cabinet garbage pull outWebGranuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of this … in cabinet filingWebDec 20, 2006 · Instead, the C2 domains, particularly the C2A domain, of exophilin4 showed markedly higher phospholipid-binding activities than those of granuphilin. The … in cabinet fanWebNov 25, 2005 · Deletion analysis showed that the syntaxin-2/3 (or Munc18-1/2)-binding site is a linker domain of Slp4-a (amino acid residues 144-354), a previously uncharacterized region located between the N-terminal Rab27A binding domain and the C2A domain. We also found that the Slp4-a.syntaxin-2 complex is actually present in rat parotid glands … in cabinet garbage racks