Phis2 酵母单杂
WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: yeast series, yeast one hybrid vector Plasmid size: 7207bp … Webb还有一点,我看了pHIS2的序列,在HIS3前面,Pmin区域里也有我的转录因子结合位点,会不会是我的转录因子结合到那里去了?不知道有没有这个可能性啊?这个minimum …
Phis2 酵母单杂
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http://muchong.com/t-9775583-1 Webb1 mars 2024 · VvACTIN or AtACTIN2 was used as internal references for grape and Arabidopsis. The qRT-PCR primers are listed in Table 1. Table 1. Gene-specific primers used for quantitative real-time PCR assays. 2.7. Transformation of Arabidopsis The 35S: VvWRKY30 vector was created by cloning genes into the pSuper1300 binary vector with …
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Webb1 apr. 2024 · The Y187 strain was prepared for the Y1H assay, we mixed pAD-CmBBX7 and pAD-GUS with pHIS2-CmFTL1pro respectively, and transformed them into Y187 strain using the lithium acetate method.Then inoculated on SD/His-Trp-Leu-plates, and screened for the lowest concentration range of 3-AT that was sufficient to inhibit the growth of … Webb12 apr. 2024 · 目的:筛选与启动子pHIS2-A互作的蛋白。 方法:以启动子A构建到酵母单杂交启动子载体pHIS2,文库质粒为pGADT7-中山杉文库。 材料: 1.启动子克隆:pHIS2-A。 根据实验需求进行扩增并酶切构建载体,两端的EcoRI和 SacI酶切位点,序列如下: GAATTCAGTTGGAATCACATCTATCATCAGAATGAAAGACCTAAGAAAAAAATGGCA …
WebbpHis2 价格: 1000元 联系方式: I47-825O-882O 立刻购买 买家导航 载体基本信息 载体质粒图谱和多克隆位点信息 载体简介 载体序列 LOCUS pHIS-2 7207 bp DNA SYN DEFINITION pHIS-2 ACCESSION KEYWORDS SOURCE ORGANISM …
Webb质粒名称:pHis2 出品公司:Clontech 质粒类型: 酵母单杂交系统 克隆方法: 多克隆位点,限制性内切酶 载体大小: 7207bp 原核抗性: Kanamycin (卡那霉素) 真核抗性:TRP1 (HIS3 selection) 备注: Clone in cis-acting sequences for use with 1-hybrid system 载体介绍 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify … flows boulderWebb1 apr. 2016 · 豆丁网是面向全球的中文社会化阅读分享平台,拥有商业,教育,研究报告,行业资料,学术论文,认证考试,星座 ... green coffee bean chlorogenic acidWebb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. flows bioWebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... flows bindingsWebb12 apr. 2024 · 方法:以启动子a构建到酵母单杂交启动子载体phis2,文库质粒为pgadt7-中山杉文库。 材料: 1.启动子克隆:pHIS2-A。 根据实验需求进行扩增并酶切构建载 … flows blood away from the heartWebb酵母单杂交体系是在酵母双杂交体系基本原理的基础上,通过观察酵母细胞内报告基因的表达状况,在酵母细胞内研究DNA与蛋白质之间的相互作用的一种技术。 许多真核生物的 … green coffee bean buyerWebb质粒名称:pHis2 出品公司:Clontech 质粒类型: 酵母单杂交系统 克隆方法: 多克隆位点,限制性内切酶 载体大小: 7207bp 原核抗性: Kanamycin (卡那霉素) 真核抗 … flows below green mountain