Raw-blue cells

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August undefined, 2024

WebFeb 17, 2024 · Coculture D1 Cells:RAW-Blue Cells Additionally, we prepared cocultures of D1 and RAW-blue cells using transwell inserts without the Dex P-A hydrogel. After 24 h incubation at 37 °C, NF-κB/AP-1 activation upon the previously mentioned stimuli was assessed by measuring the release of secreted embryonic alkaline phosphates (SEAPs) … WebSep 30, 2024 · Supernatant from the treated cells was collected and SEAP production was detected over a period of 45 minutes at 655 nm using the QUANTI-Blue ™ detection medium. Detection of protein expression by immunoblotting and densitometry. Cells from the treated well-plate were lysed and saved in the -80 ° C freezer for

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WebTherefore, RAW-Blue™ cells can be used as: TLR reporter cells: they express all TLRs, with the exception of TLR5, at different levels NOD reporter cells: … WebSep 6, 2024 · RAW-Blue and THP-1-Blue cells were plated at 1x10 6 cells/ml in flat bottom 96-well plates in a 100 µL volume. Excipients and TLR ligands were added for a final volume of 200 μL. After 24 h of stimulation, supernatants were collected and NF-κB activation was measured using the QUANTI-Blue detection medium according to manufacturer … pusta in english

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WebJun 29, 2024 · RAW-Blue macrophages were purchased from InvivoGen (San Diego, CA). All cells were propagated in RPMI 1640 supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 °C in a 5% CO 2 incubator [16, 17]. Cell viability assay. Cell death was quantified by the MTT reduction assay. Cell viability was quantified by the Alamar … WebRAW-Blue cells were treated with the shown concentrations of FP20 and incubated for 16–18 h. MPLA and LPS (100 ng/mL) were used as controls. The 100% stimulation has been assigned to the positive control LPS. Data are expressed as mean ± SEM of at least three independent experiments ... WebOct 1, 2024 · Results to characterize the IFN response time of the RAW-Blue ISG ™ (RAW-Blue) and B16-BlueIFN-α/β™ (B16-Blue) cells to IFNα and IFNβ and SEAP catalyzed Quantiblue™ (QB) development time are shown in Fig. 1, Fig. 2, Fig. 3.In Fig. 1 a range of IFN concentrations of 1 to 10,000 Unit/ml (U/ml) were added to each cell type and incubated … pusta kartka pdf

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WebRAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products. InvivoGen has developed RAW-Blue ™ cells and RAW-Lucia ™ cells, which express a secreted inducible ... WebApr 6, 2024 · RAW 264.7 was inoculated onto a 96-well plate at a density of 2x10^4 cells/well, incubated for 24 h under the above conditions. Then, I stimulated RAW with LPS (1 ug/ml) after 1 hour of drug ... pusta hrustaWebNF-κB activity from LPS-activated RAW-Blue™ cells after incubation with 30 and 300 μg/ml of acacia (AG), 30 and 300 μg/ml of guar gum (GG) and 30 μg/ml of 5ASA for 24 h in serum-free medium ... pusta kartka online

"WebRAW-blue cells were pretreated with 200μM Tamiflu, 100μg/mL MMP9i, or 100μg/mL BIM-23127 for 30min followed by 10μg/mL imiquimod or left cultured in media. Cells (1×107 cells) are pelleted ... " - Raw-blue cells

Raw-blue cells

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WebNational Center for Biotechnology Information WebDec 20, 2024 · The measured mTLR9 activity value of all the synthesized 24-mer ODNs in the dataset. The ODNs were divided into two groups of high (shown in purple) and low (shown in green) activity using a cutoff score of 0.4, based on the optimal density (OD) results from the Raw-blue reporter cell assay

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WebRAW 264.7. TIB-71 ™. RAW 264.7 is a macrophage cell line that was established from a tumor in a male mouse induced with the Abelson murine leukemia virus. Product category. WebJun 5, 2013 · RAW-Blue™ cells are permanently transfected with an NF-κB- and AP-1-sensitive promoter-driven alkaline phosphatase. NF-κB and AP-1 binds to the promoter upon nuclear translocation, and induces the expression of alkaline phosphatase that is detected by a dye-based assay and a plate reader.

WebRAW‑Dual™ cells stably express two reporter genes encoding SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase . SEAP expression depends on the activation of the endogenous MIP-2 promoter. MIP‑2, also known as CXCL2, is the murine homolog of human IL-8, a chemokine produced in an NF-κB-dependent manner [3]. WebJun 28, 2024 · QUANTI-Blue colorimetric enzyme assay: RAW-Blue cells were added at 1 × 10 5 cells per well in a 96-well plate and sequentially the same amounts of different particles at a particle: cell ratio ...

WebSep 5, 2024 · RAW-Blue cells and HEK 293 h-TLR transfected cells Murine RAW-Blue, HEK-BLUE-hTLR2 and HEK-BLUE-hTLR4 cells carrying a SEAP reporter construct were purchased from InvivoGen (San Diego, CA, USA). WebRAW-Blue™ cells were derived from the murine RAW 264.7 macrophages with chromosomal integration of a SEAP reporter construct inducible by NF-κB. RAW-Blue™ cells express many pattern-recognition receptors (PRRs), including all toll-like receptors (TLRs) except for TLR5, but with TLR4 and TLR8 expressed at the highest levels.

WebLipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. The reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to ...

WebFeb 13, 2016 · Anti-inflammatory bioassay. RAW-Blue cells (5 × 10 4 /well) were cultured in 96-well plates for 24 h, and then treated with LPS (1 μg/mL) alone or together with test samples for 20 h. Secreted embryonic alkaline phosphatase (SEAP) activity in the medium was determined by QUANTI-Blue medium following the manufacturer’s instructions. pusta książka allegroWebSize. 3-7 x 10e6 cells. Contact us. Ask RAW ™ cells selection audit. Properties. Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water. Conjugation. Blue Substrate. pusta kolox tomatoWebAug 24, 2010 · RAW-Blue cells are derived from RAW-264.7 macrophages that stably express a secreted embroyonic alkaline phosphotase (SEAP) gene which can be induced by NF-κB or AP-1 transcription factors. Treatment of RAW-Blue cells with Rx inhibited the LPS-induced NF-κB activity in a time- and dose-dependent manner as indicated by the reduced … pusta kolyskaWebRAW-Blue™ ISG cells allow the detection of bioactive murine type I IFNs or PAMPs by monitoring the activation of the interferon regulatory factor (IRF) pathway. They are derived from the RAW 264.7 macrophages. RAW-Blue™ ISG cells stably express a secreted embryonic alkaline phosphatase (SEAP) gene under the control of the interferon-stimulated pusta kartkaWebApr 22, 2015 · S2 Fig. RAW-BLUE cells detect trace levels of endotoxin in heparin lots. RAW-BLUE cells were cultured with 10 different lots of heparin (#1–10) in the presence or absence of 100pg/mL endotoxin for 24h. TNF-α levels were measured by ELISA in cell supernatant at 24 h. Each point represents mean ± SD of triplicate cell culture. pusta jakWebJan 21, 2024 · Raw-blue™ cells were cultured at 37 °C using manufacturer recommendations (InvivoGen) in a 24-well microplate until 70–80% confluence (1 × 10 5 cells/well) was reached. This cell line expressed the secreted embryonic alkaline phosphatase (SEAP) gene under the control of a promoter inducible by the transcription … pusta komórka excel pusta kuźnica